!" #$ %&'()*"+,-.%+/"+%,.0*)'.1'% - - PowerPoint PPT Presentation

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!" #$ %&'()*"+,-.%+/"+%,.0*)'.1'% - - PowerPoint PPT Presentation

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SLIDE 1

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  • Bioelectricity : Italian anatomist and physician Luigi Galvani (1780)

He showed that a electrical charge applied to the spinal nerves of a frog generates muscular spasms. Informed Alessandro Volta. He relayed the findings to the academicians in England but expanded on his own contributions to the science. He made the first observations showing a threshold potential is reached (action potential).

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SLIDE 4
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SLIDE 5

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First instance of STF recorded in crayfish muscle by Charles Richet. Results of seven excitations (stimuli), the first three do not show any significant twitches while the last four show facilitation in tension

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SLIDE 7
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Original figure from Fatt and Katz, 1953 showing potentials in crab muscle fibers in response to several current pulses. Records 2-5 and 7-9 show pulses of constant duration and varying intensity. Records 10-11 show pulses of varying duration and constant intensity. (Record 6 shows injury discharge in the muscle fiber).

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SLIDE 10
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SLIDE 11

PRESYNAPTIC

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SLIDE 13

Postsynaptic

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SLIDE 14

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SLIDE 18

PRESYNAPTIC

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SLIDE 19
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SLIDE 22
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SLIDE 23
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SLIDE 31

Methods!

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SLIDE 32
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SLIDE 34
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SLIDE 35

SERCA inhibition by Thapsigargin (10 M)!

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SLIDE 36

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SLIDE 37

Comparative physiological studies on the NCX, PMCA, SERCA in relation to synaptic transmission at Drosophila NMJs

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SLIDE 38

Methods%

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SLIDE 47

Methods!

Ionic Manipulation/ Mutational method Pharmacological Method (Drugs)

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RK!

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SLIDE 49

Dissected Prep Intact Prep

Inhibiting SERCA in Kum mutants

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SLIDE 50

Inhibiting PMCA at pH 8.8 Inhibiting NCX with low [Na+]o

Ionic Methods

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SLIDE 51

Pharmacological Inhibition

Carboxyeosin (5 M)! Thapsigargin (10 M)!

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SLIDE 52

Comparing different methods of inhibition

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SLIDE 53
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SLIDE 54

B>-04((!%+NN40$!

Treatment Crayfish NMJ Drosophila NMJ Drosophila Heart EPSP Amplitude Facilitation EPSP Amplitude Depression

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Low Na (compromising NCX)

  • NS
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  • NS
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  • NS
  • Carboxyeosin (blocking PMCA)
  • NS
  • NS
  • Heat Shock (inhibiting SERCA)
  • Thapsigargin (blocking SERCA)
  • NS
  • Inhibiting all 3 channels
  • NS
  • NS

NS

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SLIDE 55

Significance

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SLIDE 56

Intracellular recordings from Drosophila larval heart

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