Approaches to Identifying Approaches to Identifying Possible - - PowerPoint PPT Presentation

▶

Aug 06, 2023 195 likes •315 views

Approaches to Identifying Approaches to Identifying Possible Mechanisms of Kidney Possible Mechanisms of Kidney Damage Damage Mellisa Manson Mellisa Manson Virginia Comonwealth University Virginia Comonwealth University Goal of Research

SLIDE 1

Mellisa Manson Virginia Comonwealth University Mellisa Manson Virginia Comonwealth University

Approaches to Identifying Possible Mechanisms of Kidney Damage Approaches to Identifying Possible Mechanisms of Kidney Damage

SLIDE 2

Goal of Research Goal of Research

Groundwork for finding a mechanism of kidney damage Aid in understanding hypertension Aid in understanding problems in kidney function Groundwork for finding a mechanism of kidney damage Aid in understanding hypertension Aid in understanding problems in kidney function

SLIDE 3

Background Background

Function of the kidney

Normal Hypertension

Possible Markers

S1P1 Hypoxyprobe Caspase-3

Function of the kidney

Normal Hypertension

Possible Markers

S1P1 Hypoxyprobe Caspase-3

SLIDE 4

Methods Methods

Immunohistochemistry

Preservation and Slide Preparation Deparaffination Antigen Retrieval Staining

Block Serum Primary Antibody Secondary Antibody Hematoxylin

Immunohistochemistry

Preservation and Slide Preparation Deparaffination Antigen Retrieval Staining

Block Serum Primary Antibody Secondary Antibody Hematoxylin

SLIDE 5

S1P1 S1P1

Endothelial Marker Expressed all over body Role at kidney Possible Mechanisms Endothelial Marker Expressed all over body Role at kidney Possible Mechanisms

SLIDE 6

Hypoxyprobe Hypoxyprobe

Marker for hypoxia Distribution Role at kidney Possible Mechanism Marker for hypoxia Distribution Role at kidney Possible Mechanism

SLIDE 7

100x Low Salt Negative Hypoxyprobe 1:16000 Cortex Hypoxyprobe 1:32000 Hypoxyprobe 1:64000 Medulla

Papilla

SLIDE 8

Caspase-3 Caspase-3

Marker for substance essential to apoptosis Distribution Role at kidney Possible Mechanism Marker for substance essential to apoptosis Distribution Role at kidney Possible Mechanism

SLIDE 9

Cortex 100x 400x 1000x Control Low Salt Normal Diet High Salt 1000x

SLIDE 10

Conclusion Conclusion

Substances and the trends in their presence Ways to combat hypertension S1P1 receptors Hypoxia Apoptosis Substances and the trends in their presence Ways to combat hypertension S1P1 receptors Hypoxia Apoptosis