Autors: Luiza Nicolosi Guido*, Camile Castilho Fonteles, Mariana - - PowerPoint PPT Presentation

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Dec 10, 2022 239 likes •391 views

Note: for non-commercial purposes only Paternal consumption of a selenium-deficient diet programs the susceptibility of female offspring to mammary carcinogenesis Autors: Luiza Nicolosi Guido*, Camile Castilho Fonteles, Mariana Papalo Rosim,

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Paternal consumption of a selenium-deficient diet programs the susceptibility of female offspring to mammary carcinogenesis

Autors: Luiza Nicolosi Guido*, Camile Castilho Fonteles, Mariana Papaléo Rosim,

Fabia Andrade de Oliveira, Silvia Maria Franciscato Cozzolino, Thomas Prates Ong.

* Phd student at the Food Science program – Laboratory of Nutrigenomics and Programming; Department of Food and Experimental Nutrition - University of São Paulo – Brazil

Note: for non-commercial purposes only

SLIDE 2

Breast cancer incidence and mortality worldwide

SLIDE 3

Paternal programming

Male germinative cell Fetal development in early life

Risk of developing Non-communicable diseases in adulthood

CARONE et al. Cell, v. 143, p. 1084–1096, 2010; SHEAU-FANG et al. Nature,

v. 467, p. 963 – 966, 2010; RANDO. O.J. Cell, v. 151, n. 4, p. 702-708, 2012

SLIDE 4

Selenium

Source of Se

Essential for the metabolism Implication on male fertility Protective activity against breast cancer reported

ALATISE & SCHRAUZER. Biol Trace Elem Res, v. 136, p. 127–139, 2010. WHANGER, P.D. Brit J. Nutr, v. 9, p. 11–28, 2004.

SLIDE 5

Average Selenium Intake

FAIRWEATHER-TAIT et al. Antioxidants & Redox Signaling, v. 14, n. 7, 2011

SLIDE 6

Male germinative cell Breast cancer risk in adult life Mammary gland development in early life

Paternal breast cancer programming

Se deficiency or supplementation

SLIDE 7

Experimental design – Age in weeks

Approved by the ethics committee on animal use of the University of Sao Paulo – Protocol CEUA/ FCF/ 382

SLIDE 8

Paternal data

* Different compared to control; a – different compared between groups; # - marginal difference (ANOVA) – mean ± SEM p≤0,05

SLIDE 9

Female offspring data

* Different compared to control; a – different compared between groups; # - marginal difference (ANOVA) – mean ± SEM p≤0,05

SLIDE 10

Mammary epithelial growth

* Different compared to control; a – different compared between groups p ≤ 0,05 ; # - marginal difference

Nipple End of epithelial tree Limphy-node End of epithelial tree

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p ≤ 0.05 Days after DMBA administration Frequency of animals without tumor Mammary tumor incidence (first tumor appearance) of the female

ffspring from deficient (0.05ppm), control (0.15ppm) and

supplemented (1ppm) diet fathers. p ≤ 0.05 according to ANOVA. Values expressed in mean ± SEM

Mammary tumor incidence and latency

* Different compared to control; a – different compared between groups; # - marginal difference p≤0,05

* , a

SLIDE 12

Conclusion and Further Steps

The preliminary results show that paternal selenium deficiency increases the susceptibility of the female offspring to mammary carcinogenesis, while selenium supplementation has a protective

effect. Further steps will attempt to elucidate the molecular and

epigenetic mechanisms involved in this programming.

SLIDE 13

Acknowledgment

Laboratory of Nutrigenomics and Programming– FCF/USP
Laboratory of Micronutrients – FCF/USP
Laboratory of Nutrition and Cancer – FCF/USP
Laboratory of Functional Food Development– FCF/USP

Finantial Support:

Camile C. Fontelles Mariana P. Rosim Fábia O. Andrade Raquel S. Cruz Vanessa C. Pires Gabriela Costa

Thomas P. Ong