Binding of Autoantibodies in The Pathogenesis of Small Nerve Fiber - PowerPoint PPT Presentation

7.2.2020 Demonstrating Novel Binding of Autoantibodies in The Pathogenesis of Small Nerve Fiber Neuropathy Daria Keren , 6 th year GM student, University of Debrecen, Hungary Guidance by: Efrat Shavit-Stein, Ph.D, Neuroscience Research Lab Tali Drori- Snir, M.D. Neuroimmunology clinic Prof. Joab Chapman, M.D, Ph.D, Head of Neurology Department 1 Amir Dori, M.D, Ph.D, Neuromuscular clinic https://www.meresearch.org.uk/our-research/ongoing-studies/role-of-autoantibodies/

Peripheral Nervous System 2 https://www.researchgate.net/figure/The-central-nervous-system-CNS-and-peripheral-nervous-system-PNS-source_fig1_317485174

Autonomic Nervous System 3 https://images.app.goo.gl/VGQm17RvXZoUrAjK6

Autonomic Nervous System: Adrenergic Receptors 4 https://images.app.goo.gl/BDCuzrFZmZFWjGt29

Large vs. Small Nerve Fibers Nerve fibers are classified based on their thickness (in diameters) into small fibers and large fibers . This correlates directly to their speed of conduction. Source: https://www.researchgate.net/figure/Classification-of-the-peripheral-nerve-fibers_tbl1_255952315 5 https://www.researchgate.net/figure/Peripheral-nerve-anatomy-image-courtesy-of-Myoscience_fig1_304365497

Peripheral Neuropathy https://images.app.goo.gl/GCW86s13BbzoUyWBA • One of the most common neurological manifestation, though often underdiagnosed. • A manifestation of damages to peripheral nerve fibers, by different pathologies, that are characterized by impairment to sensation, motoric 6 and autonomic functions.

Sorce: https://www.mdedge.com/ccjm/article/175849/neurology/office-approach-small-fiber-neuropathy 7

Accounts for 30-50% of cases 8 Source:https://bakodx.com/epidermal-nerve-fiber-density-testing-an-overview-2/#post/0

Small Fiber Neuropathy Diagnosis Epidermal neurites, Epidermal neurites, Control healthy patient patient with small fiber neuropathy https://www.aafp.org/afp/2011/1101/p995.html jamaneurology_oaklander_2019_rv_190004 9

Autonomic System in the Skin 10 Source: https://www.frontiersin.org/files/Articles/469534/fneur-10-00970-HTML/image_m/fneur-10-00970-g001.jpg

Study the Role of Autoantibodies in Small Fiber Neuropathy After Breast Silicone Implantation • IVIG and plasmapheresis showed improvement. • Anti β 2, α 1 and muscarinic antibodies were found in these patients • May a specific binding can explains the autonomic complaints? • The major aim is to characterize the specific binding of these autoantibodies 11

The Method Immunofluorescence https://images.app.goo.gl/c8TNM8eAxw3qowhD9 https://lanlab.ee/toode/cryostat-microtome/ https://www.shutterstock.com/de/video/clip-1019518030- white-lab-mouse-albino-close-up-on-background https://images.app.goo.gl/Y1Xb 12 xxhZaHVZENye7 https://images.app.goo.gl/rcx9sx4Fbp2joADv9 https://www.banggood.com/25pcs-Biology-Glass-Prepared-Microscope-Slides-Lab-Specimens-With-Wooden-Box-p- 1124398.html

Purified IgG from a Patient with High Titer of β 2 Receptor Autoantibodies Epidermis Blood vessels 13

Purified IgG from a Patient with High Titer of α 1 Receptor Autoantibodies Epidermis Sweat Glands are stained 14

Purified IgG from a Patient with High Titer of Muscarinic Receptor Autoantibodies Epidermis Sweat Glands are stained 15

םוכיס 1 . תימונוטואהו תירפירפה םיבצעה תכרעמ תונוש תומקרו םינוש םיכילהת דוקפתל ןתמורתו ףוגב . 2 . לומל םיקד םיבצע יביס ןיב לדבההו היתפוריונ םיבע. 3 .םיקד םיבצע יביס לש היתפוריונ , ללשו הינמיס תועודיה תויגולויטאה. 4 .םיקד םיביס לש היתפוריונ ןוחבא. 5 .תירקיעה ונתרטמ : ידוחייה רשקה תא ןייפאל תולתשומ םישנב םיינומצע םינדגונ לש רועב 16 םיקד םיביס לש היתפוריונמ תולבוסה ןוקיליס.

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