Cancer chemoprevention and identification of antiangiogenic - - PowerPoint PPT Presentation

Cancer chemoprevention and identification of antiangiogenic properties of olive oil compounds

• Beneficial and preventive role of

the Mediterranean diet in the

• ccurrence of several chronic

diseases including cancer.

• These effects have partially been

explained by the consumption of

• live products which contain a

range of phenolic compounds, (natural antioxidants and highly bioavailable).

• Aim of this study was to investigate

and compare the effects of dietary compounds from virgin olive oil on endothelial cell angiogenic process.

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(Sylvie Lamy, Ph.D. / Chaire en prévention et traitement du cancer – Université du Québec à Montréal, Canada)

ROP9 Track 1 : Prevention and early detection Disclosure of interest : None declared

ERK RK pathway pathway! JNK JNK pathway pathway! P3 P38 pathway pathway! PROLIFERATION! ANGIOGENESIS! Hydroxytyrosol! Oleuropein! Oleic acid! Tyrosol! Taxifolin! VEGFR2! VEGF-A! P! P! P! P! P! P! RAF! TAK! MEKK! MEK1! MEK2! MKK4! MKK7! MKK3! MKK6! P38! JNK! ERK! MIGRATION!

ROP9 Sylvie Lamy Track 1 : Prevention and early detection Disclosure of interest : None declared

Ctl Ctl Tyr OL HT Tax OA 25 50 75 100 VEGF (1 µg/mL) (50 µM)

Lenght of tube network (% of stimulated control)

Control Control Tyrosol Oleuropein Hydroxytyrosol Taxifolin Oleic acid VEGF

A

Inhibition of endothelial capillary-like tube formation Inhibition of endothelial cell proliferation and migration

Ctl Tyr OL Tax HT OA 0.0 0.5 1.0 (50 µM)

Cell migration (X-fold of stimulated control)

Ctl Tyr OL Tax HT OA 0.0 0.5 1.0 (50 µM)

Cell proliferation (X-fold of stimulated control)

B

VEGF (25 ng/mL) VEGF (50 ng/mL) Proliferation Migration

ROP9 Sylvie Lamy Track 1 : Prevention and early detection Disclosure of interest : None declared

pVEGFR2 (Tyr1175)

Inhibition of VEGF ligand/receptor (VEGF/VEGFR2) signaling

Our study demonstrated that HT, Tax and OA are the most potent angiogenesis inhibitors and can have chemopreventive role in cancer through the inhibition of VEGFR2 pathway. These findings highlight the importance of the diet in cancer prevention.

C D

Ctl Ctl HT Tax Tyr OL OA (50 µM) VEGF (50 ng/mL)

pVEGFR2 (Tyr1214) VEGFR2 pVEGFR2 (Tyr1059) pVEGFR2 (Tyr951) VEGFR2 VEGFR2 VEGFR2

Ctl Ctl HT Ctl Ctl Tax Ctl Ctl OA VEGF VEGF VEGF Ctl Ctl HT Tax OA VEGF

pERK ERK pJNK JNK pP38 P38