Cha ra c te riza tio n o f GAGE a ntig e n e xpre ssio n in thirte - - PowerPoint PPT Presentation
Cha ra c te riza tio n o f GAGE a ntig e n e xpre ssio n in thirte e n c o mmo n b re a st c a nc e r c e ll line s Pre se nte d b y: Bre nt Vo e ls Ca nkde ska Cika na Co mmunity Co lle g e F o rt T o tte n, No rth Da ko ta Why is Bre a st
Pre se nte d b y: Bre nt Vo e ls Ca nkde ska Cika na Co mmunity Co lle g e F
Ove r the c o urse o f a life time 1 in 8 wo me n will b e dia g no se d with b re a st
c a nc e r.
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stima te s: 200,000 wo me n a re dia g no se d pe r ye a r, a lo ng with 1,700 me n. Appro xima te ly 40,000 wo me n a nd 450 me n die a nnua lly fro m b re a st c a nc e r.
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n the USA wo me n ha ve a 12% c ha nc e o f de ve lo ping inva sive b re a st c a nc e r a t so me po int in the ir live s.
BRCA1 a nd BRCA2 g e ne s a re linke d to a hig h susc e ptib ility o f b re a st
c a nc e r b ut o c c ur in o nly 5% o f b re a st c a nc e r pa tie nts.
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n 2006 the c o st o f b re a st c a nc e r tre a tme nt in the US wa s e stima te d to b e $13.9 b illio n do lla rs.
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urthe r re se a rc h ma y pro vide insig ht ino t b re a st c a nc e r pa thwa ys invo lve d in dise a se pro g re ssio n.
Cho se n fo r inve stig a tio n b a se d o n pre vio us mic ro a rra y re sults a nd
sub se q ue nt inve stig a tio n c o nduc te d a t the Unive rsity o f No rth Da ko ta .
Mic ro a rra y da ta indic a te d tha t the y we re diffe re ntia lly re g ula te d
b a se d o n the pre se nc e o r a b se nc e o f the N- a nd C-te rmina l o f MT
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he g o a l o f this re se a rc h is to c ha ra c te rize the e xpre ssio n o f GAGE fa mily a ntig e ns a c ro ss se ve ra l E R re c e pto r po sitive a nd ne g a tive b re a st c a nc e r c e ll line s.
Be lo ng to a g ro up o f c a nc e r/ te stis a ntig e ns no rma lly e xpre sse d in huma n
g e rmline c e lls.
Also fo und to b e e xpre sse d in se ve ra l tumo r type s. GAGE
a ntig e ns fa ll into thre e g ro ups.
GAGE
GAGE
s 2 thro ug h 8 a mino a c id se q ue nc e s a re 98% ide ntic a l.
GAGE
a ntig e ns ma y dire c t c e ll pro life ra tio n, diffe re ntia tio n, a nd the surviva l
No rma lly e xpre sse d in a dult ma le g e rm c e lls, a nd a sub se t o f o o c yte s in the
a dult o va ry.
Pre se nt in 26% o f b re a st c a nc e rs. Also e xpre sse d in sto ma c h c a nc e r, ne uro b la sto ma , a nd
e so pha g e a l c a nc e r.
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xpre ssio n is c o rre la te d to a po o r pro g no sis a nd a g g re ssive tumo r type .
No rma l e xpre ssio n is limite d to immune privile g e d site s ma king the m
use ful ta rg e ts fo r pro g no stic indic a to rs.
Po te ntia l ta rg e ts fo r immuno the ra py.
MCF
7: No MT
R+
T
47D: E R+
MDA-MB-157: T
riple ne g a tive (No E R, PR, o r HE R2 re c e pto rs)
MDA-MB-231: T
riple ne g a tive (No E R, PR, o r HE R2 re c e pto rs)
MDA-MB-361: 1-7HB2 I
mmo rta lize d no n-tumo r fo rming c e ll line . ZR-75-1: E
R+
ZR-75-30: E
R+
VP267: E
R-
VP303: E
R+ b ut pro g e ste ro ne re c e pto r ne g a tive
SVCT
: SV40 T ra nsfo rme d huma n b re a st e pithe lia l c e lls. No n-tumo rig e nic .
HS578T
: T riple ne g a tive .
MT
e stro g e n a nd pro g e ste ro ne re c e pto r e xpre ssio n. Dihydro te sto ste ro ne inhib its pro life ra tio n.
T 47D c e ll line sho ws c o nsiste ntly hig h e xpre ssio n No te : Sta tistic s we re no t pe rfo rme d a t this po int. T he re is no sing le c o ntro l g ro up.
GAGE 12B wa s o nly e xpre sse d in the T 47D c e ll line . T he e xpre ssio n wa s a lso lo we r tha n o the r GAGE a ntig e n e xpre ssio n in T 47D c e ll line s.
GAGE 12J ha s the hig he st e xpre ssio n le ve ls in ZR-75-1 c e lls GAGE 13 ha s the hig he st e xpre ssio n le ve ls in VP303 AND MT
GAGE 06 is e xpre sse d a t a ve ry lo w c o py numb e r in T 47D, MDA-MD-361 a nd ZR-75-01 c e ll line s.
GAGE
a ntig e n e xpre ssio n is hig hly va ria b le a mo ng st the c e ll line s te ste d .
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he re is no c le a r c o rre la tio n b e twe e n GAGE a ntig e n e xpre ssio n a nd e stro g e n re c e pto r sta tus.
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xpre ssio n o f GAGE 2B,2C,12C, a nd 12D we re hig hly e xpre sse d in T 47D c e lls.
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xpre ssio n is va ria b le a mo ng st the GAGE a ntig e ns in the c e ll line s te ste d .
Ma de po ssib le b y thre e g ra nts
(NARCH, I NBRE , I CE
I )
All g ra nts func tio n to hire ST
E M fa c ulty, a nd to c o nduc t stude nt drive n re se a rc h.
NARCH a nd I
NBRE suppo rt a te n we e k summe r re se a rc h inte rnship.
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CE
I suppo rts fa c ulty te a c hing a nd re se a rc h in ST E M.
Sinc e 2014 Ca nkde ska ha s a dde d
$250,000 wo rth o f re se a rc h e q uipme nt.
Ca nkde ska is lo o king to e xpa nd
its re se a rc h fa c ilitie s e ve n furthe r.
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stude nts.