Conservation genetics of the rare species Delphinium exaltatum (R - - PowerPoint PPT Presentation

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Conservation genetics of the rare species Delphinium exaltatum (R - - PowerPoint PPT Presentation

Jan 16, 2023 213 likes •433 views

Conservation genetics of the rare species Delphinium exaltatum (R ANUNCULACEAE ) of the Appalachian mountains and the Ozark highlands of Missouri Kelsey Huisman Dr. David Bogler Northern Michigan University Missouri Botanical Garden I

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Conservation genetics of the rare species Delphinium exaltatum (RANUNCULACEAE) of the Appalachian mountains and the Ozark highlands of Missouri

Kelsey Huisman

Northern Michigan University

  • Dr. David Bogler

Missouri Botanical Garden

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INTRODUCTION

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  • bjective

Examine the genetic variation and differentiation within and among populations of D. exaltatum from the Appalachian mountains and the Ozark highlands of Missouri to distinguish the effects of geographic isolation.

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METHODS

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study sites

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  • Habitat

– Dolomite glades with alkaline soils – An average of 65% shade – Primarily found on southwest to northwest facing slopes

  • Associated plant species

– >85% Chinkapin oak (Quercus muehlenbergii) – Hairy angelica (Angelica venenosa)

  • Encroaching plant species

– Cedar (Juniperus virginiana) – Sugar maple (Acer saccharum)

  • Cauline
  • Petiolate
  • Deeply lobed
  • Raceme of 8-30 blue to purple flowers
  • Individual

flowers are radially symmetrical with five sepals

  • One of the

sepals creates a spur

  • Height is between 4 – 6 feet,

and at times taller

life cycle

  • April-July: Vegetative
  • July-September: Flowering
  • August-October: Fruit
  • Mid to late November-March: Dormant
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extractions

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amplification

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1st PCR cycle

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gel-stab technique

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2nd PCR cycle

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sequencing

  • Yale University – DNA Analysis Facility
  • Sanger sequencing

Missouri, Howell County sample 11 – large band, forward sequence

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RESULTS

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Figure 1. Likelihood analysis. Missouri populations are shown in green.

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DISCUSSION

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ACKNOWLEDGEMENTS

  • National Science Foundation
  • National Park Service

– Daniel Drees – Christine Steinwand

  • Missouri Botanical Garden

– Dr. David Bogler – Justin Zweck – Dr. Rosa Ortiz-Gentry – Missouri Botanical Garden Staff

  • REU Students
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Questions?!