Disclosures Urine Trouble: Women's Bladder Health and the Urinary - - PDF document

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Nov 06, 2023 384 likes •469 views

9/4/2019 Disclosures Urine Trouble: Women's Bladder Health and the Urinary Microbiome 1 2 Common Urogynecologic Who is Linda Brubaker? Clinical Stories Got Me Thinking 3 4 1 9/4/2019 5 6 Detection of Bacteria (and

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9/4/2019 1

Urine Trouble: Women's Bladder Health and the Urinary Microbiome Disclosures Who is Linda Brubaker?

Common Urogynecologic

Clinical Stories Got Me Thinking

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Detection of Bacteria (and other Organisms)

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DETECTING BACTERIAL DNA

16S rRNA GENE SEQUENCING

Quick Classification Update

Bacteria Eubacteria Proteobacteria Gammaproteobacteria Enterobacteriales Enterobacteriaceae Escherichia Escherichia coli

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Are the bacteria alive?

Are “culture negative” urines truly negative?

Urobiome: there is a microbiota in the female bladder This urine is not sterile

EQUC

Blood agar 100 ul 48 hours

Standard

Blood agar 1 ul 24 hours

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Response to UUI Treatment Study

Design

20 40 60 80 100 5 10 15 20 25 30 35 40 45 50 55

Number of Unique Bacterial Species Number of Unique Participants

per individual

Women and UTI

Half of all women will get a UTI in their lifetime 30-50% experience recurrence of infection in 6-12 months 2-5% of experience recurrent UTIs (R-UTI) Mainstay of treatment is antibiotics  adverse drug events  antibiotic resistance

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Antibiotic resistance threat

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In The Future

Funding

U10 HD41250