Dynamics of OBT Accumulation in Dynamics of OBT Accumulation in - PowerPoint PPT Presentation

Dynamics of OBT Accumulation in Dynamics of OBT Accumulation in Aquatic Biota Aquatic Biota January 25-29, 2010 Sang Bog Kim, Ph.D. Research Scientist Environmental Technologies Branch Chalk River Laboratories Chalk River, Ontario Canada UNRESTRICTED / ILLIMITÉ

Background in Canada • The aquatic system has gotten less attention than terrestrial ecosystem - airborne effluent - liquid effluent • Canada has different sizes of water body - River - Lake - Ocean • Tritium leaking incident from CRL • Relate to drinking water standard UNRESTRICTED / ILLIMITÉ

Experiments • Mussel ( Elliptio complanata ) experiment: 2006 and 2007 - Whole body • Minnow ( Pimephales promelas ) experiment: 2008 - Whole body • Rainbow trout ( Oncorhynchus mykiss ) experiment: 2009 ~ - Organ distribution (muscle, liver and viscera) UNRESTRICTED / ILLIMITÉ

Mussel Experiment • Lake experiments (Perch Lake and Upper Bass Lake) - Uptake (from Ottawa River to Perch Lake) - Depuration (from Perch Lake to Upper Bass Lake) • Provided a scenario to EMRAS • Tritium uptake and depuration • Longer term experiment (Perch Creek and Upper Bass Lake) - Uptake (from Ottawa River to Perch Creek) - Depuration (from Perch Lake to Upper Bass Lake) UNRESTRICTED / ILLIMITÉ

Mussel Experiment (1) L e g e n d S a m p lin g L o c a tio n L a k e / R iv e r O ttaw a River M a in C R L la b o ra to ry s ite C R L B o u n d a ry R o a d � U p p e r B a s s L a k e P e rc h C re e k � W e ir P e rc h L a k e 0 1 2 k ilo m e tre s M a p -0 8 -0 1 3 UNRESTRICTED / ILLIMITÉ

Mussel Experiment (2) Water HTO Date of Mussel Time after Mussel Concentration Harvest transplantation (Bq/L) July 14, 2006 - <12 6,680 ± 52.3 July 15, 2006 0 hour 7,524 ± 37.2 August 14, 2006 30 days 4,460 ± 13.6 October 11, 2006 90 days 3,511 ± 76.2 May 14, 2007 300 days 9,530 ± 85.0 August 30, 2007 400 days Water concentrations varied. UNRESTRICTED / ILLIMITÉ

Mussel Experiment (3) Water HTO Date of Mussel Time after Mussel Concentration Harvest transplantation (Bq/L) 2,631 ± 14.8 July 15, 2006 (Perch Lake) 48 ± 4.9 July 15, 2006 0 hour 40 ± 6.7 August 17, 2006 30 days 53 ± 3.9 October 11, 2006 90 days 66 ± 2.5 May 14, 2007 300 days 55 ± 2.5 August 30, 2007 400 days 62 ± 2.5 October 24, 2007 467 days UNRESTRICTED / ILLIMITÉ

Mussel Experiment (4) 2500 OBT u p take OBT d ep u ration OBT concentration (Bq/L) 2000 1500 1000 500 0 0 30 90 300 400 467 Days afte r transplantation The mussel uptake experiment showed that the OBT concentration reached 7% of the HTO value 30 days after transplantation . UNRESTRICTED / ILLIMITÉ

Minnow Experiment • Experiment in the small tank (Biological Research Facility) • Uptake from the water body was about 30,000 Bq/L • Sampling time was up to 1,032 hours (43 days) - Sample was not enough to measure OBT - OBT/HTO ratio was 0.11 after 43 days exposure UNRESTRICTED / ILLIMITÉ

Minnow Experiment (1) 1.E+05 Tritium Concentration (Bq/L) 1.E+04 1.E+03 1.E+02 1.E+01 HTO OBT 1.E+00 0 1 1 2 4 6 24 48 168 840 1032 Tim e a fte r e x posure (hr) In the aquatic ecosystem, the OBT concentration in fish following an accidental HTO exposure (~30,000 Bq/L) for a month would not deliver a significant dose of tritium. UNRESTRICTED / ILLIMITÉ

Rainbow Trout Experiment • Experiment in tanks (Biological Research Facility) • OBT formation rate from HTO (7,000 Bq/L) - ICRP suggest 3-4% - Exercise 2 for EMRAS II - OBT measurements are ongoing (March 2010) • OBT formation rate from OBT-contaminated food - Similar to HTO experiment (7 days, 30 days, 70 days, 140 days) - OBT-contaminated food UNRESTRICTED / ILLIMITÉ

Rainbow Trout Experiment (1) UNRESTRICTED / ILLIMITÉ

Rainbow Trout Experiment (2) UNRESTRICTED / ILLIMITÉ

Rainbow Trout Experiment (3) UNRESTRICTED / ILLIMITÉ

Rainbow Trout Experiment (4) UNRESTRICTED / ILLIMITÉ

Rainbow Trout Experiment (5) UNRESTRICTED / ILLIMITÉ

Rainbow Trout Experiment (6) UNRESTRICTED / ILLIMITÉ

Rainbow Trout Experiment (7) UNRESTRICTED / ILLIMITÉ

Rainbow Trout Experiment (8) • Tissue free water tritium (TFWT) was extracted from the frozen samples using a specially designed freeze- drying vacuum system • Completely dried sample was grinded and rinsed with tritium depleted water (10 grams with 30 mL) • Approximately 10g of sample was loaded into a Parr bomb (Model 1121, USA) • Toluene distillation • The distilled water made up to 8 mL with tritium depleted water and mixed with 10 mL Ultima Gold XR • Counting time was 100 minutes UNRESTRICTED / ILLIMITÉ

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