Fungal Spore Morphology Estelle Levetin, PhD Disclosure No - - PowerPoint PPT Presentation

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Fungal Spore Morphology Estelle Levetin, PhD Disclosure No - - PowerPoint PPT Presentation

Nov 03, 2022 5 likes •769 views

Session 1202: Basic Aeroallergen Course Fungal Spore Morphology Estelle Levetin, PhD Disclosure No conflicts to disclose Fungal Spore Characteristics Characteristics Spore size Spore shape Number of cells Attachment Scars

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Session 1202: Basic Aeroallergen Course

Fungal Spore Morphology

Estelle Levetin, PhD

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Disclosure

  • No conflicts to disclose
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Fungal Spore Characteristics

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Characteristics

  • Spore size
  • Spore shape
  • Number of cells
  • Attachment Scars
  • Wall characteristics
  • Spore color
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Spore Size, Shape, and Septation

  • SIZE: 2mm to 100 mm
  • SHAPE: Globose, elliptical, fusiform,

asymmetric, lemon-shaped, barrel-shaped, curved

  • SEPTATION: Non-septate (one cell),

single septum, transverse septa, transverse and longitudinal septa, random septa, pseudoseptate

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Other Characteristics

  • ATTACHMENTS: Attachment scars,

attachment pegs

  • APPENDAGES
  • WALL CHARACTERISTICS: Smooth,

granular, reticulate, spines, warts, wall thickness

  • COLOR: Hyaline (colorless) to deeply

pigmented

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Globose Barrel-shaped Non-septate Random septa Lemon-shaped Club-shaped Transverse septa Attachment scars Elliptical Curved Transverse and Longitudinal septa Ornaments: spines Asymmetric and germ pore Cylindrical Pseudoseptate Appendages

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Spore color

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Asexual Spores

Also known as Deuteromycetes, Fungi Imperfecti (imperfect fungi),

  • r Mitospores
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Asexual Spores

  • Typically the most abundant spores in the

atmosphere

  • Conidia often formed on specialized hyphae

called conidiophores

  • Asexual stage of ascomycetes
  • Look for attachment scars where the spores

were attached to the conidiophore or to each

  • ther
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Cladosporium

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Note the septum

Cladosporium

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Several species of Cladosporium are common in the atmosphere

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Note the prominent attachment scars on Cladosporium conidia

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Alternaria

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Alternaria

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Curvularia

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Curvularia Nigrospora Drechslera

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Drechslera-type spores

  • Several genera of

fungi have similar cylindrical spores

– Drechslera – Bipolaris – Exserohilum – Helminthosporium

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Drechslera-type spores

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Pithomyces

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Note the colorless attachment at the base of Pithomyces spores

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Pithomyces in culture

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Epicoccum

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Epicoccum spore cluster (sporodochium)

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Penicillium species

  • Produce distinctive

conidiophores (spore bearing structures)

  • Spores are usually

spherical to oval and form in chains

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Aspergillus species

  • Produce distinctive

conidiophores (spore bearing structures)

  • Spores are usually

spherical to oval and form in chains

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Penicillium-Aspergillus type spores

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Penicillium-Aspergillus type spores

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Aspergillus niger in culture Aspergillus niger spores

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Fusarium

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Botrytis

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Oidium

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Other Asexual Spores on the NAB Count sheet

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Cercospora

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Nigrospora

Nigrospora Culture Air Sample

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Periconia

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Polythrincium Peronospora

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Stemphylium

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Torula

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Other Asexual Spores NOT on the NAB Count Sheet

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Spegazzinia

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Tetraploa

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Pestalotiopsis

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Stachybotrys

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Division Ascomycota

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Eight ascospores are produced in each ascus without any attachment scars

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Ascospores are sometimes found in groups of eight in air samples

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*Leptosphaeria ascospores

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*Chaetomium ascospores

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*Pleospora ascospores

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Diatrypella ascospores (*Family Diatrypaceae)

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Phaeosphaeria annulata

Bright field Phase contrast

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Sporomiella ascospores

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Venturia ascospores

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Paraphaeosphaeria ascospore

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Leptosphaerulina ascospore

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Various ascospores

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Many ascospores on a rainy day

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Division Basidiomycota

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Basidiospore Basidium

Attachment

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*Ganoderma basidiospores

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*Agrocybe-type *Coprinus-type

Other basidiospores that are easy to recognize

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Coprinus and Ganoderma basidiospores

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Psathyrella Psathyrella velutina Russula Stropharia

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Boletus Thelephora Galerina Chlorophylum

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Lycoperdon Calvatia Pisolithus Scleroderma

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Mixed Basidiospores

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Rust spores: Puccinia

Puccinia uredospores Puccinia teliospores

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Smut Spores

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Smut Spores

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Myxomycete (slime mold) spores

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Other slime mold spores