[PPT] - GCC Highlighted Products GSure Gel Extraction kit GSure Soil DNA PowerPoint Presentation

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GSure Gel Extraction kit GSure Soil DNA Isolation kit GSure Sputum DNA Isolation Kit GSure Stool DNA Isolation Kit GSure Urine DNA Isolation Kit GSure Yeast RNA Extraction Kit TRIZIN: Results WRTaqman 2X Master Mix GSure Bacterial genomic DNA isolation kit Gsure Ultra Nucleic Acid Isolation Kit (Plant) GSure Plant RNA Isolation Kit Fastract Direct pcr kit

GCC Highlighted Products

SLIDE 2

GSure Bacterial genomic DNA isolation kit

gDNA isolated from different no’s of Gram (+) bacteria gDNA isolated from different no’s of Gram (-) bacteria PCR was done against universal 16S primer using gDNA isolated from both Gram (+) & Gram (-) bacteria. GSure Gel Extraction kit GSure Soil DNA Isolation kit GSure Sputum DNA Isolation Kit GSure Stool DNA Isolation Kit GSure Urine DNA Isolation Kit GSure Yeast RNA Extraction Kit TRIZIN: Results WRTaqman 2X Master Mix GSure Bacterial genomic DNA isolation kit Gsure Ultra Nucleic Acid Isolation Kit (Plant) GSure Plant RNA Isolation Kit Fastract Direct pcr kit

SLIDE 3

2ml Bacterial culture Batch 1 2ml Bacterial culture Batch 2 3ml Bacterial culture Batch 1 3ml Bacterial culture Batch 2 4ml Bacterial culture Batch 1 4ml Bacterial culture Batch 2 378.7ng/ul 332.4ng/ul 547.1ng/ul 441.6ng/ul 621.4ng/ul 642.4ng/ul 314.3ng/ul 326.2ng/ul 431.2ng/ul 407.9ng.ul 651.2ng/ul 594.1ng/ul 321.2ng/ul 314ng/ul 480ng/ul 421.2ng/ul 631.4ng/ul 585.4ng/ul

100 200 300 400 500 600 700

ld

column 2ml culture New column 2ml culture

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column 3ml culture New column 3ml culture

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column 4ml culture New column 4ml culture

Series1

CELL COUNT-11.84X10^8 CELL/ ml

Comparison between two different batches with different volume of Bacterial culture

using GSure Bacterial genomic DNA isolation kit

GSure Gel Extraction kit GSure Soil DNA Isolation kit GSure Sputum DNA Isolation Kit GSure Stool DNA Isolation Kit GSure Urine DNA Isolation Kit GSure Yeast RNA Extraction Kit TRIZIN: Results WRTaqman 2X Master Mix GSure Bacterial genomic DNA isolation kit Gsure Ultra Nucleic Acid Isolation Kit (Plant) GSure Plant RNA Isolation Kit Fastract Direct pcr kit

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GSure Gel Extraction kit

Gel recovery of 2kb pcr product after extraction with GSure gel extraction kit Gel recovery of 4kb pcr product after extraction with GSure gel extraction kit Gel recovery of 6kb pcr product after extraction with GSure gel extraction kit

GSure Gel Extraction kit GSure Soil DNA Isolation kit GSure Sputum DNA Isolation Kit GSure Stool DNA Isolation Kit GSure Urine DNA Isolation Kit GSure Yeast RNA Extraction Kit TRIZIN: Results WRTaqman 2X Master Mix GSure Bacterial genomic DNA isolation kit Gsure Ultra Nucleic Acid Isolation Kit (Plant) GSure Plant RNA Isolation Kit Fastract Direct pcr kit

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Isolation of total DNA from four different soil (50mg), Total yield is plotted accordingly Minimum no of soil microbes presence can be identified by PCR using Gsure soil DNA isolation kit.

GSure Soil DNA Isolation Kit

GSure Gel Extraction kit GSure Soil DNA Isolation kit GSure Sputum DNA Isolation Kit GSure Stool DNA Isolation Kit GSure Urine DNA Isolation Kit GSure Yeast RNA Extraction Kit TRIZIN: Results WRTaqman 2X Master Mix GSure Bacterial genomic DNA isolation kit Gsure Ultra Nucleic Acid Isolation Kit (Plant) GSure Plant RNA Isolation Kit Fastract Direct pcr kit

SLIDE 6

Fig. : AGE image of 16s PCR products

+ : positive control 1 : Buccal swab sample 1; 2 : Buccal swab sample 2; 3 : Buccal swab sample 3; 4 : Mucous sample 1; 5 : Mucous sample 2; - : negative control; L : 1 kb ladder

L 5 4 3 2 1 +

L

5 4 3 2 1 +

Fig. : AGE image of human β-actin PCR

products + : positive control 1 : Buccal swab sample 1; 2 : Buccal swab sample 2; 3 : Buccal swab sample 3; 4 : Mucous sample 1; 5 : Mucous sample 2;

: negative control;

L : 100 bp ladder

1.5 kb 612 bp

GSure Sputum DNA isolation kit

GSure Gel Extraction kit GSure Soil DNA Isolation kit GSure Sputum DNA Isolation Kit GSure Stool DNA Isolation Kit GSure Urine DNA Isolation Kit GSure Yeast RNA Extraction Kit TRIZIN: Results WRTaqman 2X Master Mix GSure Bacterial genomic DNA isolation kit Gsure Ultra Nucleic Acid Isolation Kit (Plant) GSure Plant RNA Isolation Kit Fastract Direct pcr kit

SLIDE 7

L 5 4 3 2 1

Fig. : AGE image of k-ras PCR

products 1 : Buccal swab sample 1; 2 : Buccal swab sample 2; 3 : Buccal swab sample 3; 4 : Mucous sample 1; 5 : Mucous sample 2; - : negative control; L : 100 bp ladder

113 bp

Fig. : AGE image of TB PCR products

1 : Buccal swab sample 1; 2 : Buccal swab sample 2; 3 : Buccal swab sample 3; 4 : Mucous sample 1; 5 : Mucous sample 2; - : negative control; L : 100 bp ladder

1 2 3 4 5 L -

112 bp

GSure Sputum DNA isolation kit

GSure Gel Extraction kit GSure Soil DNA Isolation kit GSure Sputum DNA Isolation Kit GSure Stool DNA Isolation Kit GSure Urine DNA Isolation Kit GSure Yeast RNA Extraction Kit TRIZIN: Results WRTaqman 2X Master Mix GSure Bacterial genomic DNA isolation kit Gsure Ultra Nucleic Acid Isolation Kit (Plant) GSure Plant RNA Isolation Kit Fastract Direct pcr kit

SLIDE 8

L S2 S1 S1 S2 + L

1.5 kb
Fig. : AGE image of 16s PCR

products + : positive control

: negative control;

L : 1 kb ladder

Fig. : AGE image of genomic

DNA S1 : sample 1 S2 : sample 2; L : 1 kb ladder

GSure Stool DNA Isolation Kit

GSure Gel Extraction kit GSure Soil DNA Isolation kit GSure Sputum DNA Isolation Kit GSure Stool DNA Isolation Kit GSure Urine DNA Isolation Kit GSure Yeast RNA Extraction Kit TRIZIN: Results WRTaqman 2X Master Mix GSure Bacterial genomic DNA isolation kit Gsure Ultra Nucleic Acid Isolation Kit (Plant) GSure Plant RNA Isolation Kit Fastract Direct pcr kit

SLIDE 9

GSure Stool DNA Isolation Kit

S1 S2 L +

Fig. : AGE image of Human

GAPDH PCR products + : positive control

: negative control;

L : 100 bp ladder

S1 S2 L

113 bp

114 bp

Fig. : AGE image of k-ras PCR

products

: negative control;

L : 100 bp ladder GSure Gel Extraction kit GSure Soil DNA Isolation kit GSure Sputum DNA Isolation Kit GSure Stool DNA Isolation Kit GSure Urine DNA Isolation Kit GSure Yeast RNA Extraction Kit TRIZIN: Results WRTaqman 2X Master Mix GSure Bacterial genomic DNA isolation kit Gsure Ultra Nucleic Acid Isolation Kit (Plant) GSure Plant RNA Isolation Kit Fastract Direct pcr kit

SLIDE 10

GSure Urine DNA Isolation kit

Checking the minimum amount of samples for kit 1 2 3 4 5 6 7 8 9 1 2 3 4 5 6 7 8

Fig. 37 : AGE image of PCR products using

Human GAPDH primers. ( 30 cycles) Lane 1 : Positive control Lane 2 : 500µl; Lane 3 : 750µl; Lane 4 : 1ml; Lane 5 : 2ml; Lane 6 : 3ml; Lane 7 : 4ml; Lane 8 : Negative control; Lane 9 : 100 bp ladder.

Fig. 38 : AGE image of PCR products using

Human GAPDH primers. (30 cycles) Lane 1 : Positive control Lane 2 : 250µl; Lane 3 : 100µl; Lane 4 : 75µl; Lane 5 : 50µl; Lane 6 : 25µl; Lane 7 : 100 bp ladder. Lane 8 : Negative control;

114bp 114bp

GSure Gel Extraction kit GSure Soil DNA Isolation kit GSure Sputum DNA Isolation Kit GSure Stool DNA Isolation Kit GSure Urine DNA Isolation Kit GSure Yeast RNA Extraction Kit TRIZIN: Results WRTaqman 2X Master Mix GSure Bacterial genomic DNA isolation kit Gsure Ultra Nucleic Acid Isolation Kit (Plant) GSure Plant RNA Isolation Kit Fastract Direct pcr kit

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GSure Yeast RNA Extraction Kit

1 2 3 L 5 6 7 STARTING CELL NUMBER : 1 and 5–1X108 2and6-5X108 3and7-10X108 CELL: Yeast Elution volume : 50ul LOAD- 4ul L-1KB Ladder  YEAST RNA, that could be visualised on the gel can be isolated using this kit is 1X 108 cells

Fig. : AGE image of yeast RNA

GSure Gel Extraction kit GSure Soil DNA Isolation kit GSure Sputum DNA Isolation Kit GSure Stool DNA Isolation Kit GSure Urine DNA Isolation Kit GSure Yeast RNA Extraction Kit TRIZIN: Results WRTaqman 2X Master Mix GSure Bacterial genomic DNA isolation kit Gsure Ultra Nucleic Acid Isolation Kit (Plant) GSure Plant RNA Isolation Kit Fastract Direct pcr kit

SLIDE 12

GSure Yeast RNA Extraction Kit

PCR amplification No of cells used for RNA isolation Lane 1: 1X108 Lane 2: 5X108 Lane 3: 10X108 L-100bp Ladder Primers(fungal 18S) used for cDNA synthesis using isolated 1ul RNA from each sample L 1 2 3

420 bp

GSure Gel Extraction kit GSure Soil DNA Isolation kit GSure Sputum DNA Isolation Kit GSure Stool DNA Isolation Kit GSure Urine DNA Isolation Kit GSure Yeast RNA Extraction Kit TRIZIN: Results WRTaqman 2X Master Mix GSure Bacterial genomic DNA isolation kit Gsure Ultra Nucleic Acid Isolation Kit (Plant) GSure Plant RNA Isolation Kit Fastract Direct pcr kit

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AGE image of isolated RNA Lane1&2: CELL line Lane3&4: Monocot Leaf L: 100bp ladder SAMPLE:1.HEK CELLS 2. HELA cell STARTING SAMPLE MATERIAL:1.5 million Load-4ul SAMPLE: 3.Grass, 4. Wheat STARTING SAMPLE MATERIAL:25mg Load-4ul

TRIZIN: Results

GSure Gel Extraction kit GSure Soil DNA Isolation kit GSure Sputum DNA Isolation Kit GSure Stool DNA Isolation Kit GSure Urine DNA Isolation Kit GSure Yeast RNA Extraction Kit TRIZIN: Results WRTaqman 2X Master Mix GSure Bacterial genomic DNA isolation kit Gsure Ultra Nucleic Acid Isolation Kit (Plant) GSure Plant RNA Isolation Kit Fastract Direct pcr kit

SLIDE 14

TRIZIN: Results

SAMPLE:RICE SEED( Lane: 1,2,3) AND RICE LEAF( Lane 4,5,6) STARTING SAMPLE MATERIAL: 25mg Load-4ul

Fig: AGE image of isolated RNA from seed and Rice leaf (in comparison to TRIZOLE) Lane1&4: using Triazole,Lane2,3 ,5&6: TRIZINE ,L: 100bp ladder 1 2 3 L 4 5 6

GSure Gel Extraction kit GSure Soil DNA Isolation kit GSure Sputum DNA Isolation Kit GSure Stool DNA Isolation Kit GSure Urine DNA Isolation Kit GSure Yeast RNA Extraction Kit TRIZIN: Results WRTaqman 2X Master Mix GSure Bacterial genomic DNA isolation kit Gsure Ultra Nucleic Acid Isolation Kit (Plant) GSure Plant RNA Isolation Kit Fastract Direct pcr kit

SLIDE 15

Fig: AGE image cDNA of plant Primer-rbcl Lane1:L6(+RT), Lane2:L6(-RT) L-100bp ladder L 1 2 550bp 550bp Fig: AGE image cDNA of seed Primer-rbcl Lane1:L6(+RT), Lane2:L6(-RT) L-100bp ladder L 1 2

Quality checking of isolated RNA using TRIZIN

GSure Gel Extraction kit GSure Soil DNA Isolation kit GSure Sputum DNA Isolation Kit GSure Stool DNA Isolation Kit GSure Urine DNA Isolation Kit GSure Yeast RNA Extraction Kit TRIZIN: Results WRTaqman 2X Master Mix GSure Bacterial genomic DNA isolation kit Gsure Ultra Nucleic Acid Isolation Kit (Plant) GSure Plant RNA Isolation Kit Fastract Direct pcr kit

SLIDE 16

Fig : Gapdh specific Amplification Plot using 1ng/ul of Template Fig : Gapdh specific Amplification Plot

using 0.1ng/ul of Template

WRTaqman 2X Master Mix( UNG Based)

Real Time PCR Results

GSure Gel Extraction kit GSure Soil DNA Isolation kit GSure Sputum DNA Isolation Kit GSure Stool DNA Isolation Kit GSure Urine DNA Isolation Kit GSure Yeast RNA Extraction Kit TRIZIN: Results WRTaqman 2X Master Mix GSure Bacterial genomic DNA isolation kit Gsure Ultra Nucleic Acid Isolation Kit (Plant) GSure Plant RNA Isolation Kit Fastract Direct pcr kit

SLIDE 17

Fig : Amplification Plot using 0.01ng/ul of Template Fig 53 : Amplification Plot using 0.001ng/ul of Template

WRTaqman 2X Master Mix( UNG Based)

Real Time PCR Results

GSure Gel Extraction kit GSure Soil DNA Isolation kit GSure Sputum DNA Isolation Kit GSure Stool DNA Isolation Kit GSure Urine DNA Isolation Kit GSure Yeast RNA Extraction Kit TRIZIN: Results WRTaqman 2X Master Mix GSure Bacterial genomic DNA isolation kit Gsure Ultra Nucleic Acid Isolation Kit (Plant) GSure Plant RNA Isolation Kit Fastract Direct pcr kit

SLIDE 18

WRTaqman 2X Master Mix( UNG Based)

Real Time PCR Results

1 2 3 4 5 6 7 8 9

Fig 54 : AGE Image of Control GAPDH Real Time PCR Product Lane 1,2: PCR Product using and 1ng/ul template Lane 3,4: PCR Product using and 0.1ng/ul template Lane 5,6: PCR Product using and 0.01ng/ul template Lane 7,8: PCR Product using and 0.001ng/ul template Lane 9: 100bp DNA Ladder GSure Gel Extraction kit GSure Soil DNA Isolation kit GSure Sputum DNA Isolation Kit GSure Stool DNA Isolation Kit GSure Urine DNA Isolation Kit GSure Yeast RNA Extraction Kit TRIZIN: Results WRTaqman 2X Master Mix GSure Bacterial genomic DNA isolation kit Gsure Ultra Nucleic Acid Isolation Kit (Plant) GSure Plant RNA Isolation Kit Fastract Direct pcr kit

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Sampl e (RNA) Conc(ng/ul) 260/280 260/230 1. 796.4 2.04 2.20 2. 609.1 2.19 2.22 3. 665.8 2.20 2.24 4. 596.6 2.10 1.48 Sample (DNA) Conc(ng/u l) 260/280 260/230 1. 175 1.85 2.09 2. 174 1.86 2.08 3. 173.9 1.85 1.99 4. 173.8 1.84 1.84

Fig 12 : AGE image of Plant RNA using ultra kit for plant.

Lane1-4 : Isolated from brassica plant L : 100bp ladder

1 2 3 4 5 L

1 2 3 4 L

RNA gDNA

Gsure Ultra Nucleic Acid Isolation Kit (Plant)

GSure Gel Extraction kit GSure Soil DNA Isolation kit GSure Sputum DNA Isolation Kit GSure Stool DNA Isolation Kit GSure Urine DNA Isolation Kit GSure Yeast RNA Extraction Kit TRIZIN: Results WRTaqman 2X Master Mix GSure Bacterial genomic DNA isolation kit Gsure Ultra Nucleic Acid Isolation Kit (Plant) GSure Plant RNA Isolation Kit Fastract Direct pcr kit

SLIDE 20

GSure Ultra Nucleic Acid Isolation Kit (Plant)

Quality checking of isolated RNA

1 2 3 4 5 6 7 8 L N

Lane 1 : Using 250ng template RNA (+RT) Lane 2 : Using 250ng template RNA (-RT) Lane 3 : Using 250ng template RNA (+RT) Lane 4 : Using 250ng template RNA (-RT) Lane 5 : Using 500ng template RNA (+RT) Lane 6 : Using 500ng template RNA (-RT) Lane 7 : Using 500ng template RNA (+RT) Lane 8 : Using 500ng template RNA (-RT) L : 100bp ladder N : NTC

550bp

Fig 14 : AGE image of cDNA based PCR using isolated RNA. GSure Gel Extraction kit GSure Soil DNA Isolation kit GSure Sputum DNA Isolation Kit GSure Stool DNA Isolation Kit GSure Urine DNA Isolation Kit GSure Yeast RNA Extraction Kit TRIZIN: Results WRTaqman 2X Master Mix GSure Bacterial genomic DNA isolation kit Gsure Ultra Nucleic Acid Isolation Kit (Plant) GSure Plant RNA Isolation Kit Fastract Direct pcr kit

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SAMPLE CONC ng/lambda 260/280 260/230 Batch 1 RNA-A 1178.0 2.13 2.41 Batch 1 RNA-B 400.9 2.12 2.37 Batch 2 RNA-A 959.8 2.13 2.48 Batch 2 RNA-B 263.0 2.09 2.23 Batch 3 RNA-A 1235.8 2.14 2.42 Batch 3 RNA-B 239.1 2.09 2.38

1ST KIT-3 MONTHS OLD 2ND KIT-4 MONTHS OLD 3RD KIT-1 MONTHS OLD

Part-A Part-B Part-A Part-B Part-A Part-B

Batch 1 Batch 2 Batch 3

GSure Plant RNA Isolation Kit

GSure Gel Extraction kit GSure Soil DNA Isolation kit GSure Sputum DNA Isolation Kit GSure Stool DNA Isolation Kit GSure Urine DNA Isolation Kit GSure Yeast RNA Extraction Kit TRIZIN: Results WRTaqman 2X Master Mix GSure Bacterial genomic DNA isolation kit Gsure Ultra Nucleic Acid Isolation Kit (Plant) GSure Plant RNA Isolation Kit Fastract Direct pcr kit

SLIDE 22

Fastract Direct pcr kit Amplification from different stage of Rice plant

GSure Gel Extraction kit GSure Soil DNA Isolation kit GSure Sputum DNA Isolation Kit GSure Stool DNA Isolation Kit GSure Urine DNA Isolation Kit GSure Yeast RNA Extraction Kit TRIZIN: Results WRTaqman 2X Master Mix GSure Bacterial genomic DNA isolation kit Gsure Ultra Nucleic Acid Isolation Kit (Plant) GSure Plant RNA Isolation Kit Fastract Direct pcr kit

SLIDE 23