SLIDE 1
Honey Bees and Na,ve Prairie Flowers
Speaker: Morgan Carr-Markell Co-authors on the work presented: Cora Demler, ScoC Cornman, Deborah Iwanowicz, and Marla Spivak MHBA Mee,ng October 11, 2016
Honey Bees and Na,ve Prairie Flowers Speaker: Morgan Carr-Markell - - PowerPoint PPT Presentation
Honey Bees and Na,ve Prairie Flowers Speaker: Morgan Carr-Markell - - PowerPoint PPT Presentation
Honey Bees and Na,ve Prairie Flowers Speaker: Morgan Carr-Markell Co-authors on the work presented: Cora Demler, ScoC Cornman, Deborah Iwanowicz, and Marla Spivak MHBA Mee,ng October 11, 2016 Big Ques)on Landscape Food Colony Health
SLIDE 2
SLIDE 3
Specific Ques)ons
Background: – Many groups want to plant bee-friendly na,ve prairie flowers and restore na,ve biodiversity Ques)ons:
- 1. Can prairies be a major source of food?
- 2. What are the most-used na,ve prairie flowers?
SLIDE 4
Methods: Field sites
Four field sites around Minneapolis/St. Paul
SLIDE 5
Methods: Field sites
General Mills 1 acre Prairie Restora,on 50 acres Belwin Conservancy 300 acres Northfield- Carleton College 70 acres
SLIDE 6
Methods: Collec)ons
Mixed pollen loads Unripe honey
SLIDE 7
Methods- Microscopy
- We use the structure of pollen grains under the microscope
Microscope slide of pollen sample
SLIDE 8
Methods- DNA Sequencing
- We use the sequence of pollen DNA
DNA in nucleus Exine DNA in nucleus
DNA Microscopy
Deb Iwanowicz USGS, Leetown WV ScoC Cornman USGS, Fort Collins CO
SLIDE 9
Methods: Sequencing pollen
Integrate two methods: Propor,ons of species
Species IDs from sequencing
(Image: Cornman et al. 2015 Fig 2)
Propor,ons of pollen types
SLIDE 10
Methods: Decoding waggle dances
- Collected one of each color/direc,on combina,on
- Decoding methods- Couvillon et al. 2014
- Pollen forager dances decoded by Cora Demler
SLIDE 11
Implica)ons/Future direc)ons
- 1. Largest contributors of pollen to honey bees non-
na,ve, but certain na,ve species contribute
- 2. Plan,ng studies will help determine the effect of
density and abundance of flowers
- 3. Adding to DNA sequence databases may help
resolve taxa further
SLIDE 12
Thank you!!
Advisor: Marla Spivak CommiJee:
- Dan Cariveau
- Diane Larson
- Emilie Snell-Rood
Advice/help:
- Fitz BogoC
- Ron Bowen
- Vaughn Bryant
- Margaret Couvillon
- Ann Fallon
- George Heimpel
- Clint OCo
- Tom Rabaey
- Roger Schürch
- Anh Tran
Bee Lab/Bee Squad Field sites:
- Belwin Conservancy
- Carleton College
- Prairie Restora,ons
- Werth Farm
Funding:
- General Mills
- Na,onal Honey Board
- North Dakota
Beekeepers Associa,on
- Costco Wholesale
Corpora,on
- Project Apis m.
- Madhava Sweetners
SLIDE 13
References
- 1. Cornman RS, OCo CR V, Iwanowicz D, Pefs JS (2015)
Taxonomic characteriza,on of honey bee (Apis mellifera) pollen foraging based on non-overlapping paired-end sequencing of nuclear ribosomal loci. PloS ONE. 10:
- e0145365. doi: 10.1371/journal.pone.0145365
- 2. Couvillon MJ, Riddell Pearce FC, Harris-Jones EL, Kuepfer AM,
Mackenzie-Smith SJ, Rozario LA, Schürch R, Ratnieks FLW (2012) Intra-dance varia,on among waggle runs and the design of efficient protocols for honey bee dance decoding.
- Biol. Open 1: 467–472. doi: 10.1242/bio.20121099