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iGESM: intelligent Gene)cally Engineered Slot Machine
Our aim
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Automa0c device for random recombina)on Inspired by the adap)ve immune system
iGESM: i ntelligent G ene)cally E ngineered S lot M achine 1 Our - - PowerPoint PPT Presentation
iGESM: i ntelligent G ene)cally E ngineered S lot M achine 1 Our - - PowerPoint PPT Presentation
iGESM: i ntelligent G ene)cally E ngineered S lot M achine 1 Our aim Automa0c device for random recombina)on Inspired by the adap)ve immune system 2 Inspired by nature Random recombina)on of gene segments (V, D, J) Billions of an)bodies 3
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Random recombina)on of gene segments (V, D, J) Billions of an)bodies
Inspired by nature
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Recreate this system in bacteria
- 1. Random recombina)on between modules
- 2. Stops aEer produc)ve recombina)on
COUNTLESS combina)ons & each cell ONE product
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iGESM‐project
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Inverted repeats INVERSION
Site‐specific recombina0on
Direct repeats DELETION
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Bacteriophage Mu Inver)ble G‐segment
Gin recombina)on depends on recombina)onal enhancer!
Gin recombinase
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Gin equa0on
= +
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Periplasm (AmiC) Nucleoid (HU) Cell poles (GroES) Green (GFP) Red (mRFP) Blue (CFP)
A‐modules B‐modules
Modular design
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The recombinator construct
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A‐modules
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B‐modules
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Automa0c shut off
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Expected outcome
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Results
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Workflow
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Workflow
you are only limited by your imagina)on !!!
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High diversity out of a few modules Shuts off aEer produc0ve recombina)on Many applica)ons
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