Interfacing Nanoparticles to Biology Kimberly Hamad-Schifferli - - PowerPoint PPT Presentation

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Dec 22, 2022 142 likes •209 views

Interfacing Nanoparticles to Biology Kimberly Hamad-Schifferli Biological and Mechanical Engineering, MIT Understanding and Synthesis of nanoparticles with Nanoscale interfaces manipulation of particle unique properties with proteins and

SLIDE 1

Interfacing Nanoparticles to Biology

Kimberly Hamad-Schifferli Biological and Mechanical Engineering, MIT

S N H

3 +

S NH3

N S

+H3N

S NH3

S N H

3 +

Nanoscale interfaces with proteins and DNA Synthesis of nanoparticles with unique properties Multifunctional particles f f1 f2 Understanding and manipulation of particle heating

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Triggering drug release

SLIDE 2

Simple molecules <1nm IBM PowerPC 750 Microprocessor 7.56mm×8.799m 6.35×106 transistors semiconductor nanocrystal (CdSe) 5nm

10-10 10-5 10-9 10-7 10-6 10-8 10-4 10-3 10-2 m

Circuit design Copper wiring width 0.2µm red blood cell ~5 µm (SEM) DNA proteins nm bacteria 1 µm Nanometer memory element 1012 bits/cm2 (1Tbit/cm2)

Nanoscale Interfaces to Biology

SOI transistor width 0.12µm diatom 30 µm

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Controlling Biology with Nanoparticles

Making an interface that preserves biological function (quantify) Exploit physical properties of inorganic nanoparticles

Heating by fields
Fluorescence + Magnetic

Target function of biological molecules and processes

Multifunctional particles
Drug Delivery
Imaging
Gene expression

SLIDE 4

Enhancing Antisense Gene Regulation with NPs

Use NPs to enhance antisense by steric blockage
Study conformation of DNA on NP

~7nm NP with DNA 14mers ribosome ~20nm

S. Park, K. A. Brown, K. Hamad-Schifferli, Nano Lett., 2004
S. Park and K. Hamad-Schifferli, J. Phys. Chem. C, 2008
K. A. Brown, S. Park, K. Hamad-Schifferli, J. Phys. Chem. C, 2008

no protein protein antisense strand (DNA 15-20mer) ribosome mRNA AUG protein AUG + protein antisense strand with NP no protein AUG

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Characterizing NP-protein Interfaces

NP ligand positive/negative/neutral improve/worsen protein folding? NP material Different affinity for different residues? NP size effect of curvature on protein folding? Labeling site Sites sensitive to protein stability?

M.-E. Aubin, D. G. Morales, K. Hamad-Schifferli., Nano Lett., 2005 M.-E. Aubin-Tam and K. Hamad-Schifferli., Langmuir, 2005 M.-E. Aubin-Tam, H. Zhou, K. Hamad-Schifferli., Soft Matter, 2008 M.-E. Aubin-Tam and K. Hamad-Schifferli, Biomed Mater, 2008

MD simulations (Wonmuk Hwang, TAMU) molecular interactions

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Nanoparticles as multifunctional therapeutic agents

Inefficient conjugation
Large
Stability and biocompatibility

Au8-PAMAM particles: ~3nm Au8:

Fluorescence
MRI

Polyamidoamine dendrimer

Delivery
Functionalizable

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Carrier for drugs Imaging (multimodal) targeting

T1 T2 Untreated 60nM 120nM