The trick in biology is to select, from the thousands of facts that - - PowerPoint PPT Presentation

The trick in biology is to select, from the thousands of facts that you know, the three that are relevant to your problem, and to not be disturbed that they contradict each other. Eric Wieschaus (paraphrased)

Mark Voorhies 3D Structures

3D Structures

Mark Voorhies 4/6/2012

Mark Voorhies 3D Structures

Orienting ourselves

s e l e c t a l l c o l o r grey hide e v e r y t h i n g show cartoon Mark Voorhies 3D Structures

Orienting ourselves

2CHT → C → by chain → by chain

Mark Voorhies 3D Structures

Pick one trimer in the unit cell

s e l e c t a l l hide e v e r y t h i n g s e l e c t trimer1 , chain a+b+c show cartoon , t r i m e r 1

• r i e n t

t r i m e r 1 Mark Voorhies 3D Structures

Orient ourselves on the primary structure

trimer1 → C → by chain → chainbows

Mark Voorhies 3D Structures

Show Conolly solvent accessible surface

show s u r f a c e , t r i m e r 1 Mark Voorhies 3D Structures

Run a custom coloring script

@ p r o p e r t i e s . pml Mark Voorhies 3D Structures

Find the ligands

s e l e c t a l l hide e v e r y t h i n g show cartoon , t r i m e r 1 s e l e c t p r o t e i n , not het s e l e c t ligand , het and not r e s n hoh show s t i c k , l i g a n d Mark Voorhies 3D Structures

Orient on one active site

s e l e c t ligA , r e s i 201

• r i e n t

l i g A Mark Voorhies 3D Structures

Find the active site residues

s e l e c t g1 , p r o t e i n w i t h i n 5.0

• f

l i g A s e l e c t g2 , b y r e s g1 show s t i c k , g2 Mark Voorhies 3D Structures