April 21, 2016 4MGEN14 contamination Sleuthing the problem 4MGEN14 - - PowerPoint PPT Presentation

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April 21, 2016 4MGEN14 contamination Sleuthing the problem 4MGEN14 - - PowerPoint PPT Presentation

Nov 21, 2022 36 likes •554 views

ABC IAC April 21, 2016 4MGEN14 contamination Sleuthing the problem 4MGEN14 seed overwinter Lewisetta Rap Lynnhaven 5K 5K 5K split on site split on site split at VIMS 2n 14 10 2015 distribution 4MGEN14 seed overwinter Lewisetta

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ABC IAC April 21, 2016

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4MGEN14 contamination Sleuthing the problem

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4MGEN14 seed

  • verwinter

Rap Lewisetta Lynnhaven 5K 5K 5K

split on site split on site 2n split at VIMS

10 14 2015 distribution

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4MGEN14 seed

  • verwinter

Rap Lewisetta Lynnhaven 5K 5K 5K

split on site split on site 2n split at VIMS

10 14

58%

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A B C D E F 4MGEN13 — 40/41 98% 23/25 92% — — 20/20 100% 4MGEN14 30/32 80% — 14/15 93% 43/43 100% 21/23 91% 5/7 71% All certs so far 2016 176/186 (95%) 2015 345/375 (92%) 2014 327/332 (99%) 2013 371/385 (96%) 2012 428/480 (89%) 2016 CERTIFICATIONS to date

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Brood stock quality Industry based research

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Glycogen and Fecundity

  • NIRS technology
  • Oyster Seed Holdings
  • LOLA, DEBY.13, DEBY.14
  • Glycogen at time zero and just prior to conditioning

– Different treatments prior to conditioning

  • Fecundity/egg counts on spawning day
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Growth

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Egg count vs. Glycogen

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Egg Count vs. Length

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False winter and Dermo

  • Belief that false winter aids in conditioning and “resets”

Dermo if present

  • H° : oysters put through false winter will have lower

prevalence and intensity of Dermo compared to those not put through false winter

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DEBY.LYN.13

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Mackin Scale 0 – rare 1 – very light 2 – light 3 – moderate 4 – moderate/heavy 5 – heavy

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RFTM results

false wintered 72% prevalence weighted prevalence: 1.28 (n=25) no false winter 68% prevalence weighted prevalance: 1.32 (n=25 No difference between treatments

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Update on status of VIMS research hatchery No planning until: July 1, 2017 / Start building: July 1, 2018

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building concept

concept views

nort h

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building concept

concept views

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design .

entrance perspective

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int erior design.

interior perspectives- lobby

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int erior design.

interior perspectives- transition

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Interior Perspectives

int erior design.

interior perspectives- lower level

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int erior design.

interior perspectives- lower level

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revised f irst second plan .

programming floor plans- level 2

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revised f irst f loor plan .

programming floor plans- level 1

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North side

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South side

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Hatchery floor

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Questions about the planning process

What role industry?

  • Determining research capabilities
  • e.g., carbonate chemistry?
  • e.g., conditioning studies?
  • e.g., closed systems?

Research hatchery manager?

  • Oversee facility, part of AQ Program
  • PI for hatchery related research
  • “Journeyman” with experience
  • e.g., Alan Barton

Expertise in design?

  • VA industry?
  • Other sources of expertise
  • Donaldson
  • Meritt
  • Davis
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Family deployment 2016

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2N Deployment 2016

  • 49 low salinity families

– 25 also deployed in York River*

  • 50 high salinity families

– 15 also deployed in Lewisetta* * some families perform well in both environments

  • long line – York River & Cambridge
  • rack & bag – Lynnhaven & Lewisetta
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  • Hungars Creek

– Cherrystone – 30 families (15 high, 15 low, 2 reps per) – rack and bag – Do ABC families perform differently in the creek environments of the Eastern Shore?

  • loggers in place to measure temp. & salinity

2N Deployment 2016

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3n mortality research update Grad student: Joseph Matt Post doc: Eric Guévélou

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“The relationship between reproduction and mortality in triploid Crassostrea virginica: a matter of economic importance”

tetraploid diploid male 4n VA 4n LA 2n VA 2n ME female 2n VA VVV LLV VV MV 2n ME VVM LLM VM MM 4 triploid groups 4 diploid “controls”

Spawned in March 2015 – all groups now “ideal” size

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Deployment sites

Control

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Data from triploid mortality 2016

  • Triploid reproductive effort
  • Environment
  • Physiology
  • Growth
  • Mortality
  • Condition
  • NIRS
  • Glycogen
  • (Lipid)
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Microbiome – BK Song, Allen, Carnegie

Profiling the microbiome composition of hatchery reared oyster larvae Sea Grant PD funds Summer 2015

The totality of microorganisms and their collective genetic material present in a particular environment.

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Sampling ontogeny across hatcheries

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march april may june july august

Sampling seasonal variation across hatcheries

GP KAC OSH WOC

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  • 0.5
  • 0.4
  • 0.3
  • 0.2
  • 0.1

0.1 0.2 0.3

  • 0.4
  • 0.3
  • 0.2
  • 0.1

0.1 0.2 0.3

PV-1 PV-3 V-1 V-3 D-1 D-3 D-2 D-1 D-2 V-1 V-2 V-3 D-3 PV-2 D-2 PV-2 D-1 PV-3 PV-2 D-3 V-2 D-2 V-1 V-3 PV-3 D-1 PV-1 V-2 D-3 V-3

PC1 - Percent Variation Explained – 9.7% PC1 - Percent Variation Explained – 7.9% KAC GP WOC OSH

V-1

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D-stage V-stage PV-stage

Predominant genus found in adult

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New crop of OATs Other training

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2016 OATs

(32 applicants)

3 ‘regular’ OATs

  • Imani Black
  • Track record with Bay interests
  • Lacrosse star, ODU
  • Graduates May ‘16
  • Lizzy Profita
  • Experience in shellfish, algae
  • Americorps – Cape Cod shellfish
  • Prefers field to lab
  • Haley Ladeau
  • Experience at Milford
  • Experience at AIC (Rutgers, Cape May)
  • Extrovert!

1 ‘research assistant’ OAT

  • Chris Rubino
  • Internships at several hatcheries
  • Familiar with the field
  • Not afraid of “unflattering jobs”
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2016 OAT applicants

(placements)

  • Nick Booth – Cherrystone intern
  • Carlos Santos – Mobjack Bay Seafood
  • James McCarthy – Lynnhaven
  • Ellis Chapman – Cherrystone
  • Diamond in the rough
  • Gretchen Davis – Northern Neck
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2016 OAT Jr

(test program)

MathScience Innovation Center, Richmond

  • 2 week summer aquaculture course – “Tank to Table”
  • Tilapia
  • Trout
  • Oysters
  • Select four top students (work ethic) – oyster internship
  • 2 to VIMS for one week
  • Another 2 to VIMS for one week
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VIMS Aquaculture Program Industry Advisory Committee redefined?

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Stan Jess GP KAC 2n field 4n pre-2015 Jess FTE ABC Aquaculture @ VIMS “five” years Stan Jess KAC 2n field 4n GP (asst. director) 2015 Succession at ABC (first shown June 2016)

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INFRASTRUCTURE – VIMS Aquaculture Program AP Director

  • Brings research interest
  • Coordinates aquaculture research at VIMS
  • Administers rapid response funds

ABC BK Song Kim Reece Juliette Smith Ryan Carnegie Elizabeth Shadwick Iris Anderson Jan McDowell Roger Mann

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ENDOWMENT – VIMS Aquaculture Program AP Director

  • Brings research interest
  • Coordinates aquaculture research at VIMS
  • Administers rapid response funds

ABC BK Song Kim Reece Juliette Smith Ryan Carnegie Elizabeth Shadwick Iris Anderson Jan McDowell Roger Mann

$$

$ $

Discretionary funding

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Shellfish Industry Advisory Committee – VIMS Aquaculture Program AP Director

  • Brings research interest
  • Coordinates aquaculture research at VIMS
  • Administers rapid response funds

ABC BK Song Kim Reece Juliette Smith Ryan Carnegie Elizabeth Shadwick Iris Anderson Jan McDowell Roger Mann

$$

$ $

Discretionary funding