Genetic and molecular control of Apple tree tolerance to soil water - - PowerPoint PPT Presentation

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Genetic and molecular control of Apple tree tolerance to soil water - - PowerPoint PPT Presentation

Jan 06, 2023 130 likes •234 views

Genetic and molecular control of Apple tree tolerance to soil water stress AFEF-AGAP CIRAD-SupAgro Montpellier Lamia Krichen Agreenskill incoming fellowship (September 1st, 2014 - August 31th, 2015) Objectives Understand the mechanisms of

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Genetic and molecular control of Apple tree tolerance to soil water stress

AFEF-AGAP CIRAD-SupAgro Montpellier Lamia Krichen Agreenskill incoming fellowship (September 1st, 2014 - August 31th, 2015)

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Understand the mechanisms of response to water deprivation in apple tree (2 genotypes: GS and ST) Detect the difference of behaviour for the two genotypes GS and ST and different organs from each genotype (apex, leaf, root) Identify candidate genes for tolerance to water stress in apple tree

Objectives

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1/ Analyze the differencially expressed genes 2/ I dentify biological and molecular functions triggered under water stress 3/ I dentify key genes related to important biological function 4/ I dentify candidate genes

From transcriptome to candidate genes: How to proceed

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Sampling Well watered conditions (T0, T1, T2) Moderate Water deficit T1= 50% FTSW Severe Water deficit T2= 20% of FTSW

Experiment-sampling

apex leaf root

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Analyze of the differencially expressed transcripts

■ AryANE micro-array apple chip (126,022 genes) sense probes ⇒ 63,011 genes

UMR IRHS- INRA ANGERS (Celton et al., 2014)

Data filtering

12,901 genes differentially expressed transcripts (DET) (20.47% sense probes)

1/ AryANE micro-array validation 2/ Biological and molecular functions triggered under water stress conditions

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1/ AryANE micro-array validation

  • 20 candidate gènes selected
  • Specific primers designed
  • Quantitative RT-PCR

performed

  • Specific RT-PCR product

analysed (LightCycler 480)

R² = 0.962 R²=0961 R²=0957 R²=0884 R²=0.835

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1. Hormones 2. Sucrose-starch synthesis and degradation 3. Glycolysis 4. Cell wall 5. Ions and water transport 6. Signaling 7. Regulation of transcript 8. Abiotic stress

2/ Biological and molecular functions

Genes ontologies involved in water stress for GS and ST genotypes in roots (AgriGO & Mapman softwares)

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NCED (9-cis-epoxycarotenoid dioxygenase) CCD (carotenoid clivage) VDE (Violaxanthin de-epoxidase) β-carotene Zeaxanthin Violaxanthin Xanthoxin ABA NCED3 = the key enzyme involved in ABA biosynthesis ST NCED3↑ ST↑ ST NCED4↓ ST CCD8 ↑ GS CCD1 ↑ GS NCED5↑ GS NCED3↑

ABA biosynthesis

  • DET related to Mechanism of ABA biosynthesis under

moderate water deficit in roots

  • ABA = stress hormone
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Thanks to

Architecture et fonctionnement des espèces fruitières (AFEF) UMR amélioration génétique et adaptation des plantes méditerranéennes et tropicales (AGAP)