Gypsophila Confined Field Trial at Naivasha Waturu CN, Ngugi CN, - - PowerPoint PPT Presentation
Experiences with Genetically Modified Gypsophila Confined Field Trial at Naivasha Waturu CN, Ngugi CN, Muchui MN, Mbaka JN, Kimani AW and Muhonja L Presented at the National Biosafety Conference, KICC-Nairobi Tuesday 11 th -14 th August 2014
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Experiences with Genetically Modified Gypsophila Confined Field Trial at Naivasha
Waturu CN, Ngugi CN, Muchui MN, Mbaka JN, Kimani AW and Muhonja L
Presented at the National Biosafety Conference, KICC-Nairobi Tuesday 11th -14th August 2014
P.O. Box 220, Thika, Kenya Email: karithika12@gmail.com Charles.Waturu@kari.org
Presentation Outline
Project Title
paniculata for Colour Stability and Environmental Impact
Background Information
now commercially grown as a cut flower.
paniculata
cases light pink flowers.
bouquets
Background Information
ranging from dark purple and red to light pink through introduction of Pap1 gene.
preceded by generation of biosafety data
Gypsophila paniculata varieties for biosafety and pink colour stability.
Species Name and Habitat
Species name: Gypsophila paniculata L. Common Name: Baby's breath Herbaceous perennial plant grown as an annual crop in hotter climates
Modified Trait
Conventionally,
Gypsophila is available in predominately one colour- white Very limited varieties and limited climate conditions also light pink Introduced gene Pap1 (Production
Anthocyanin Pigment 1) is a Myb transcription factor Known to regulate the production
phenylpropanoid, including anthocyanin pigments
Gene Donor and Vector
Gypsophila varieties are genetically engineered for constitutive expression of the Pap1 gene isolated from Arabidopsis thaliana Binary transformation vector pCGN1599 Binary plasmid doesn't contain oncogenic genes which lead to the pathogenesis of the natural agrobacterium
Research Themes
Scientists and Research Themes
Research Scientist Expertise Research Theme(s) 1 Dr CN Waturu/Cecilia Ngugi Entomologist/ Biotechnologist
Effect of Crude extract of GM
Gypsophila
infectivity and development of Entomopathogenic Nematodes
Below ground effect of transgenic
Gypsophila on EPNs
Effect of transgenic Gypsophila on
aerial and ground arthropods diversity
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Scientists and Research Themes
Research Scientist Expertise Research Theme(s) 2 Dr J. Mbaka Plant Pathologist
Effect of transgenic Gypsophila on
rhizosphere bacterial/fungal and nematode communities populations 3 Dr D. Gikaara/ Dr M. Muchui/Ms. Linah Muhonja Soil Scientist/ Floriculturist/ Post-harvest Physiologist
yield/quality performance
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Scientists and Research Themes
Research Scientist Expertise Research Theme(s) 5 Ms. C Taracha Molecular Biologist
Field assessments of pollen-mediated
gene flow (PGF) from transgenic to non transgenic Gypsophila 6 MS S. Wambua Economist
Economic evaluation of the transgenic
and non transgenic Gypsophila 7 MS A Kimani ICT
Role of ICTs for Effective Dissemination
and Knowledge
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Site Preparation and Planting
Trial Site
Distribution of Gypsophila in Naivasha
GM Gypsophila CFT Site
Site Preparation
Seedlings Transportation
GM Gypsophila Seedlings
GM Gypsophila Planting
Pests and Natural Enemies
Beneficial Arthropods
Transgenic Conventional
Pot EPN Studies
Effect of Plant Extract/ EPN Studies
Gene Flow Studies
Postharvest Studies
Soil Microbial Studies
Cutworm Damage Weed infestation Diseased plants
Acknowledgements
KARI-Thika