THE SCIENCE OF NOVACQ TM IN PENAEUS MONODON Ha Truong, Tansyn Noble - - PowerPoint PPT Presentation

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Thailand 14 active Novacq ponds Australia - Yamba active Novacq ponds THE SCIENCE OF NOVACQ TM IN PENAEUS MONODON Ha Truong, Tansyn Noble and Omar Mendoza-Porras 31 st July 2019 | APFA Brisbane Convention and Exhibition Centre SUSTAINABLE

SLIDE 1

THE SCIENCE OF NOVACQTM IN PENAEUS MONODON

Ha Truong, Tansyn Noble and Omar Mendoza-Porras

SUSTAINABLE AQUACULTURE PRODUCTION

31st July 2019 | APFA Brisbane Convention and Exhibition Centre

Ridley and CSIRO alliance

Australia - Yamba active Novacq ponds

Thailand 14 active Novacq ponds

SLIDE 2

Outline

Ridley and CSIRO alliance Novacq and other marine bioactives on:

1. Growth under different feeding regimes
2. Gut microbiome
3. Health markers

2 | Science behind NovacqTM

SLIDE 3

Three post-docs focusing on

Nutrition
Immunity/health
Proteomics

NovacqTM: A novel aquafeed additive that uses natural microbial processes to increase growth, feed efficiency and health, and reduce reliance on fishmeal

Relatively low in protein and high in carbohydrate and ash
Greater than 30% increase in weight gain in monodon in tanks

Ridley and CSIRO science alliance

3 | Science behind NovacqTM

SLIDE 4

Marine ingredients

4 |

Krill, squid, prawn meal

Strategic ingredients in prawn

feed

Improves growth, intake and feed

conversion efficiency

NovacqTM

Source of microalgae, yeast,

bacteria, living and dead organic matter, carbon, minerals

Improves growth, feed efficiency

and survival

Science behind NovacqTM

Mechanisms?

Feed attractant Nutrient utilisation Meeting a deficiency Complete source of micronutrients Unknown bioactives

SLIDE 5

Communal growth trial

10 prawns/tank; 4 tanks

reps/dietary treatment

Iso-nitrogenous and energetic

diets offered to communally- housed juvenile black tiger prawns for 6 weeks

5 |

Dietary treatment

g/kg Fishmeal Krill meal Krill hydrolysates Novacq™ Fishmeal 300 210 210 300 Novacq 100 Krill meal 100 Krill hydrolysate 100 Soybean 300 300 300 300 Gluten 70 70 70 70 Wheat flour 295 285 285 195 Fish oil 15 11 11 16 Micro- ingredients 19 19 19 19

Fed at satiation (120-140%) or restricted (70%)

Science behind NovacqTM

SLIDE 6

Growth under different feeding regimes

SLIDE 7

Growth results

Growth effect of marine bioactives | Dr Ha Truong 7 |

1 2 3 4 5 6 7 8 9

Control Krillmeal Krill hydrolysate NovacqTM Control Krillmeal Krill hydrolysate NovacqTM Satiation Restricted

Weight gain (g)

NovacqTM bioactivity was enhanced when unrestricted-fed

86%

SLIDE 8

Growth results

8 |

0.5 1 1.5 2 2.5 3 3.5

Control Krillmeal Krill hydrolysate NovacqTM Control Krillmeal Krill hydrolysate NovacqTM Satiation Restricted

FCR (feed:gain)

NovacqTM obtained the best FCR
Restricted-fed diets were more efficient but growth was poor

Growth effect of marine bioactives | Dr Ha Truong

38%

SLIDE 9

Nutrient retention
Apparent nutrient digestibility
Protease and amylase activity
Intake rate
Gut evacuation rate
Behavioural attraction assessment

Other parameters measured:

9 | Growth effect of marine bioactives | Dr Ha Truong

SLIDE 10

Gut microbiome

Tansyn

SLIDE 11

Why look at the microbiome?

Microbiome linked with numerous health

conditions.

Right balance between beneficial and non-

beneficial bacteria.

Diet influences microbiome
Know diets especially Novacq improves

growth and nutrient efficiency in prawns.

Is this improved performance linked with

having a unique microbiome?

Used DNA sequencing to identify the

bacterial community present.

Presentation title | Presenter name 11 |

SLIDE 12

Prawn gut microbial profile

12 |

Highly diverse with 1435 different bacteria

types found

Diets have similar bacterial composition
Varying levels of vibrios
Broad-scale clustering based on similarity
No clustering of samples due to diet
Clear separation between the two feeding

regimes

Microbiome changes to feed | Dr Tansyn Noble

SLIDE 13

A closer look at some taxa

13 |

Both feeding regime and diet significantly influence specific taxa abundance.

Microbiome changes to feed | Dr Tansyn Noble

Diet Abundance

SLIDE 14

Haemolymph health markers

Omar

SLIDE 15

Advanced understanding of prawn health and immunity using proteomics

Evaluate the effect of diet, environmental or pathogen challenge

Developing blood health markers by proteomics | Dr Omar Mendoza-Porras 15 |

Innate Immunity

Simultaneous quantification
f multiple target proteins

currently 50 and increasing

Application across various

tissues

Non-destructive blood sample
Functionally relevant to

prawn biology Antioxidant Capacity Oxygen Transport Stress Response Reproduction and Development Growth and metabolism

SLIDE 16

Broad effects of Novacq

Developing blood health markers by proteomics | Dr Omar Mendoza-Porras 16 |

Control Novacq Novacq Control Novacq Novacq

Consistent separation of dietary treatments Unrestricted fed cohort

SLIDE 17

Expression of health markers

Developing blood health markers by proteomics | Dr Omar Mendoza-Porras 17 |

Novacq Novacq Control

0 .0 0 0 .0 5 0 .1 0 0 .1 5 0 .2 0 0 .2 5 0 .0 0 .2 0 .4 0 .6 0 .0 0 .2 0 .4 0 .6

a b b a b a a a a

Higher expression means that homeostasis is not optimal. Energy potentially diverted into metabolic adjustments rather than growth.

SLIDE 18

Agriculture and Food Ha Truong Post doctorate fellow e ha.truong@csiro.au Tansyn Noble Post doctorate fellow e tansyn.noble@csiro.au Omar Mendoza Porras Post doctorate fellow e

mar.mendozaporras

@csiro.au

AGRICULTURE & FOOD

THANK YOU ANY QUESTIONS?

Special thanks

Ridley

Dr Matt Briggs
Dr Viviana Lazzarotto

CSIRO

Dr Cedric Simon
Dr Nick Wade
Dr James Wynne
Dr Artur Rombenso
BIRC and QBP staff