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in vivo Imaging Using Nanoparticles
Department of Nuclear Medicine & Cancer Research Institute Seoul National University College of Medicine
in vivo Imaging Using Nanoparticles Keon Wook Kang, MD, PhD - - PowerPoint PPT Presentation
in vivo Imaging Using Nanoparticles Keon Wook Kang, MD, PhD - - PowerPoint PPT Presentation
in vivo Imaging Using Nanoparticles Keon Wook Kang, MD, PhD Department of Nuclear Medicine & Cancer Research Institute Seoul National University College of Medicine Failure of Nanomedicine MAY 2016 Nanoparticle Imaging in Nuclear
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Nanoparticle Imaging in Nuclear Medicine
Liver scan Bone marrow scan Lymphoscintigraphy
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Just after injection 1hr after injection 2hr after injection
PET/CT
Biodistribution of nanoparticles
NIR Imaging Before injection 1 h after injection 3 h after injection
30 nm gold nanoparticles in a mouse 30 nm silica nanoparticles in a mouse
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125I-labeled cyclic RGD peptide-
gold nanoparticle
In vivo SPECT/CT imaging of in a U87MG tumor bearing mouse tumor tumor tumor
125I-gold
nanoparticles
125I-cRGD-gold
nanoparticles Cold blocking test Small 2011
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FDA approved nano-drugs
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60 min 2 h 24 h 4 h 5 min 10 min 15 min 30 min
99mTc-albumin scan in human
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0.5 h 4 h
Capan1
24 h 13 h
99mTc-albumin scan in mouse
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Liver targeting: Exploiting the asialoglycoprotein receptor for drug delivery to hepatocellular carcinomas
- The asialoglycoprotein receptor is expressed in ~ 80 % of
hepatocellular carcinomas.
- Felix Kratz in cooperation with Prof. Luigi Fiume and Dr. G. Di
Stefano
Fiume et al.: J. Hepatology 43, 645-652, 2005; Di Stefano et al., Europ.J. Pharm. Sciences 23,393-397, 2004; Di Stefano et al., Liver Int. 24, 246-252, 2004; Di Stefano et al., Digestive and Liver Disease 35, 428-33, 2003.
HSA
S
Galactose
20 6
L-HSA-DOXO-EMCH
OH OH OH OH O O O O O OH N H N O N O O NH2
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Hep3B HT29 HepG2
64Cu-HSA 64Cu-L8-HSA 64Cu-L56-HSA
46 h after injection
64Cu-lactoamine-albumin PET
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Versatile and Finely Tuned Albumin Nanoplatform based on Click Chemistry
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Galactose-HSA Mannose-HSA HSA 0 h 4 h 2 h 24 h 48 h
64Cu labeled Albumin Platform
PET imaging
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GSA - Cy3 MSA - FNR-648
Hepatocyte vs Kupffer cell
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SPARC in cell lines
RT-PCR
Glioma cells SPARC Beta-actin
western blot
Glioma cells
43kDa
SPARC knock down cell
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in vivo fluorescent imaging
0 h 4 h 8 h 24 h
U87MG 6.0 x 6.3 mm2 U87MG SPARC KD 6.0 x 5.9 mm2
HSA-FNR648 injection via tail vein
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U87MG U87MG
- SPARC KD
*V: blood vessel
ex vivo fluorescent imaging
Theranostics 2019
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Targeting biomarker In vivo molecular imaging Precision theranostics Genomics Nano-carrier
NH2
Precision nanomedicine using molecular imaging
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Nuclear Medicine, SNU
Faculties: 22
MD: 12 / PhD: 10 (Physics, Chemistry, Molecular Biology)
Researchers: 42
DS Lee JM Jeong SE Kim KW Kang WW Lee JS Lee YK Kim JC Paeng YS Lee HY Lee HW Youn SW Oh DW Hwang
MD PhD
YH Kim GJ Cheon MG Song BC Lee HY Choi