Our team Lucas Alan Carlos Jlio Fernando Marianna Paola - - PowerPoint PPT Presentation

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Our team Lucas Alan Carlos Jlio Fernando Marianna Paola - - PowerPoint PPT Presentation

Sep 10, 2022 322 likes •816 views

Our team Lucas Alan Carlos Jlio Fernando Marianna Paola Raphael talo Clara Priscila Liza Omar Santuza Where we came from Minas Gerais, Brazil Where we came from Belo Horizonte - MG Where we came from Universidade Federal de

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Fernando Liza Santuza Omar Alan Júlio Paola Lucas Ítalo Raphael Carlos Marianna Priscila Clara

Our team

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Minas Gerais, Brazil

Where we came from

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Where we came from

Belo Horizonte - MG

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Where we came from

Universidade Federal de Minas Gerais (UFMG)

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Acute Coronary Syndrome (ACS)

NIH Medline Plus Winter 2009 Issue: Volume 4 Number 1

A worldwide problem

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Acute Coronary Syndrome (ACS)

A worldwide problem

WHO, 2013

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Acute Coronary Syndrome (ACS)

A Brazilian problem

Teich et al., Rev Bras Cardiol., 2011

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Acute Coronary Syndrome (ACS)

A challenge

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  • To develop a sensitive device capable of

detecting and integrating the signals of ACS biomarkers, setting a prognosis. Aiming high

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Biomarkers

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Tang et al., N Engl J Med., 2013

Trimethylamine-N-Oxide

TMAO: Introduction

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‘

Trimethylamine-N-Oxide

TMAO: Construction

TorS TorT

TMAO

TorT

TorR TMAO TorR*

pTorCAD RFP BBa_K1086000

  • ------- Ba_K1086002 -------
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‘

Trimethylamine-N-Oxide

TMAO: Construction

TorS TorT

TMAO

TorT

TorR TMAO TorR*

pTorCAD RFP TorT + TMAO 2 TorT + 2 TMAO + TorS

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Trimethylamine-N-Oxide

TMAO: Modeling

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Trimethylamine-N-Oxide

TMAO: Results

TorCAD+RFP ----- TMAO

Fluorescence/Absorbance

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Ischemia Modified Albumin

IMA: Introduction

Normal patient blood (Cobalt added) Ischemic patient blood (Cobalt added) Albumin IMA

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Ischemia Modified Albumin

IMA: Construction

pRCNA YFP

  • ------- Ba_K1086001 --------

Normal patient blood (Cobalt added) Ischemic patient blood (Cobalt added) Albumin IMA

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Ischemia Modified Albumin

IMA: Modeling

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Ischemia Modified Albumin

IMA: Modeling

Albumin 90 µM

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Ischemia Modified Albumin

IMA: Modeling

Cobalt 25 µM

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Ischemia Modified Albumin

IMA: Results

RCNA+YFP ----- Cobalt

Free Cobalt Assay: Fluorescence/Absorbance

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Ischemia Modified Albumin

IMA: Results

Bovine Serum Albumin Assay: Fluorescence

RCNA+YFP ----- Cobalt

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Ischemia Modified Albumin

IMA: Results

RCNA+YFP ----- IMA+Cobalt

Ischemic and Non-Ischemic Mice Sera Assay: Fluorescence

Non-Ischemic Ischemic

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Talking with specialists

Human Practices

Doctor Antonio Luiz Pinho Ribeiro

ETHICS BIOSAFETY

Neuza Antunes Rodrigues

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Interest over time. Web Search. Worldwide, 2004 - present. Scientific dissemination

Human Practices

Interest over time. Web Search. Worldwide, 2004 – present English x Portuguese

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Goals

Human Practices

Cardbio Impact

Thinking

  • utside

the box Talking with specialists Collaboration Scientific dissemination

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Scientific dissemination

Human Practices

Our team

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Scientific dissemination

Human Practices

Our team Our university

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Scientific dissemination

Human Practices

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Scientific dissemination

Human Practices

Our team Our university Public schools

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Scientific dissemination

Human Practices

UFMG & Escolas

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Scientific dissemination

Human Practices

Missions

  • 1. Fuel from sunlight
  • 2. Microplastic hazard on the oceans
  • 3. Spoiled meat
  • 4. Malaria and artemisin
  • 5. Celiac disease
  • 6. Space exploration
  • 7. Our own project

The Brickard Game

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Scientific dissemination

Human Practices

Our team Our university Public schools Our state

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Scientific dissemination

Human Practices

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Scientific dissemination

Human Practices

Our team Our university Public schools Our state Brazil

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Scientific dissemination

Human Practices

Website

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Scientific dissemination

Human Practices Wikipedia

facebook.com/igemufmg twitter.com/igemufmg

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Scientific dissemination

Human Practices

Video about Synthetic Biology Newspaper

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Goals

Human Practices

Cardbio Impact

Thinking

  • utside

the box Talking with specialists Collaboration Scientific dissemination

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Thinking outside the box

Human Practices Hackspace

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Thinking outside the box

Human Practices

Our song!

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Goals

Human Practices

Cardbio Impact

Thinking

  • utside

the box Talking with specialists Collaboration Scientific dissemination

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Collaboration

Human Practices

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Collaboration

Human Practices

Jamboré – Meeting of Brazilian teams

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Achievements

 Assembly, registry and characterization of new biobricks:

 RCNA + YFP (BBa_K1086001)  TorCAD (BBa_K1086000)  TorCAD+ RFP (BBa_K1086002)

 Modeling of our systems.  Expand the knowledge about synthetic biology in our University,

  • ur State and our Country.

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Thanks

Sponsors Other supporters

Laboratório de Genética Bioquímica Laboratório de Sinalização Celular e Nanobiotecnologia Laboratório de Genômica e Genética de Parasitos Laboratório de Imunoquímica de Toxinas Naturais Laboratório de Sistema de Informação Laboratório de Angiogênese Professor Gastone Castellani Professora Andréa Mara Macedo Neuza Antunes Rodrigues Juliana Pimenta Rosalvo Fogaça –UFPR Izabella Pena Neshich – UNICAMP Doutor Antonio Luiz Pinho Ribeiro André Luis Martins Reis – Johns Hopkins

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Thanks

Crowd funding donators

Denis Roger Pedro Henrique Nascimento de Aguiar Filipe Silva Villela Sabrina Lima Marina Diniz Diego Pessoa Santos Graciela Kunrath Lima Bel Bitar Isabela F. Prates Marcela Segatto do Carmo Henrique Teichmann Nicole Scherer Wagner Arbex Glória Franco Naíla Barbosa da Costa Fernanda Santos Freitas Pedro E. C. Vieira Francislon Silva de Oliveira Lucas Antunes de Siqueira Costa Pedro Pinho Teixeira Jarbas Rohr Mainá Bitar Leonardo Lucas Carnevalli Dias Luandy Baião Wesley Júnio da Silva Michele Araújo Neuza Antunes Juliano Leal Ceres Luciana Alves Maíra Freire Amanda Campelo

Thank you!